Objective: Target customers who want to observe bright fluorescence of specimens under stereo microscopes, such as customers who study nematodes, zebrafish, fruit flies, embryos, and mice
--- Reproduction, Development
--- Functional analysis of genes, proteins and other biomolecules
Olympus Solution-MacroView MVX10
New application areas-macro fluorescence imaging and macro fluorescence screening
Macro fluorescence image
- Users can count target expressions without the need to fix barley and zebrafish (live cell observation)
- Users can check targets expressed on mouse brain slices at low magnification, and can detect the details of nerve cells.
2. Macro fluorescence screening
- You can see clearer details and reduce screening omissions.
- There is no need to re-examine with a special high-power microscope.
MVX10 Product Concept
- Bright fluorescence under a macro microscope
- Designed for high-quality imaging
MV (Parallel Focal Length) Objectives for MVX
| WD || NA |
| NA |
| MVPLAPO 0.63X || 87 || 0.15 || 0.078 |
| MVPLAPO 1X || 65 || 0.25 || 0.125 |
| MVPLAPO 2XC || 20 || 0.50 || 0.25 |
MVPLAPO2XC (NA: 0.5) is equipped with an optical calibration ring to calibrate imaging errors when observing living cells in liquid (can correct errors caused by cell culture fluid up to 5mm thick, directly observe adherent cells)
- Parfocal distance: 137mm
- Connection: M65 x 1.5 mm thread
- MVPLAPO2XC is equipped with an optical calibration ring to calibrate imaging errors when viewing living cells in liquid
- Two-hole conversion objective turntable optional: MVX-2RE
High S / N signal-to-noise ratio, excellent fluorescence image.
UIS2 objectives have excellent fluorescence signal-to-noise ratio (S / N). This technology not only improves the numerical aperture, but also reduces autofluorescence. The current technology has been able to effectively detect the very weak fluorescence excited by weak excitation light, so that the UIS2 system can perform excellent fluorescence imaging of living cells.
High-quality fluorescent excitation block for fluorescent protein
U-MGFPHQ / XL and other highly sharpened coatings and high transmittance (90% -95%) can effectively transmit the fluorescence emitted by fluorescent proteins. In this way, even with weak excitation light, a bright image can be observed, at the same time, fluorescence attenuation is prevented, and the possibility of cell damage is reduced.
High-performance equipment makes image recording more flexible and efficient. <br style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; padding-top: 0px"> Even if the sample emits weak light, The coaxial illumination fluorescent unit still provides clear and bright observation.
This reflective fluorescent lighting unit can be used to observe the fluorescence of living cells in a stereo microscope. Its high-performance fluorescence filter has a sharp cut-off value and high permeability, and can even capture the weak luminescence of fluorescent proteins.
Application of MVX10 <br style="padding-bottom: 0px; margin: 0px; padding-left: 0px; padding-right: 0px; padding-top: 0px"> When MVX10 was launched, OLYMPUS visited Europe, Japan, In the United States and other places, there are more than 30 experts in research fields such as catfish, zebrafish, nematodes, fruit flies, and a series of experiments have been conducted in cooperation with them.
The main application areas are:
Fluorescence Image: Brain Slice
- You can observe very weak brain slice fluorescence that cannot be seen with a conventional stereo microscope.
- Protein distribution can be traced.
| MVX10 main technical parameters |
| Mirror body MVX-ZB10 || Zoom ratio || 10 (0.63-6.3) |
| Observation tube |
| Field of view || twenty two |
| Lens tilt angle || 0-23 degrees adjustable |
| Optical path switching || 2 stops (eyepiece / photograph: 100/0 and 0/100) |
| Epi-fluorescence device |
| Lighting method || Coaxial epitaxy |
| Fluorescence filter dial || 4 hole rotation, 3 excitation block positions + bright field position |
| light source || 100W mercury lamp room and transformer, or 75W xenon lamp room and transformer |
| Intermediate zoom device |
| Magnification || 1X, 2X switching |
| Objective lens (when eyepiece is WHN10X) || MVPLAPO0.63X || MVPLAPO1X || MVPLAPO2XC |
| Comprehensive magnification || 4.0X-40X || 6.3X-63X || 12.5X-125X |
| Working distance || 87 || 65 || 20 |
| Numerical aperture || 0.15 || 0.25 || 0.5 |
| Field of View (mm) || 55.4-5.5 || 34.9-3.5 || 17.4-1.7 |
| Lighting stand || shelf || Advanced transmitted light illuminator SZX-ILLB2 |
Transmitted light illuminator SZX-ILLK
Bright and dark field transmitted light illumination device SZX-ILLD2
Large stand SZX-STL
| Focusing device || MVX-FOF: coarse and fine focus device |
SZX-FOA: Motorized Focusing Device